Antibacterial Prevalence of Multidrug Resistant Pseudomonas Aeruginosa from Critically Ill Patients

Abstract

Background: Pseudomonas aeruginosa, an adaptive pathogen leads to broad-spectrum infection in human. The treatment of infection is difficult as Pseudomonas aeruginosa is innately imperious to numerous antibiotics.

Objective: Therefore, the aim of this study is to investigate multiple drug resistance (MDR) pattern of Pseudomonas aeruginosa.

Methods: For this purpose, laboratory based positive samples from different sources including blood, urine, peritoneal fluid and tracheal swabs were selected and then processed for the isolation of MDR Pseudomonas aeruginosa. Bacterial spp. was isolated and purified by using standard microbiological practices. Purified cultures were processed for the bacterial identification by using different staining techniques, morphological, physiological and biochemical analysis. Final characterizations was performed, on the basis of 16S rRNA analysis. For this purpose, genomic DNA was extracted and 16S rRNA primers designed. Gene amplification of 16S rRNA was performed with the help of 16S rRNA primers in TaqMan polymerase chain reaction. Attribute of MDR was assessed by performing antibiotic drug sensitivity reaction.

Results: Antibiotic reveled the resistance of Pseudomonas aeruginosa against multiple drugs. For instance, 47% Pseudomonas aeruginosa were resistant to carbimipenem, 46% were resistant to meropenem, 37% were resistant to tazobactam, 56% were resistant to ciprofloxacin, 49% were resistant to gentamycin, 44% were resistant to cetazidmine and 53% were resistant to levofloxacin. Results showed lowest resistance towards tazobactam and highest to ciprofloxacin. The attribute of highest resistance against ciprofloxacin was further evaluated by gene amplification of ciprofloxacin resistant Pseudomonas. Aeruginosa. Gene amplification revealed the 90% ciprofloxacin resistant strains contained the ParC gene.

Conclusions: In this study, we concluded that selected Pseudomonas aeruginosa showed high resistant pattern against ciprofolxacine and carbapanam, which indicated Pseudomonas aeruginosa as life threatening pathogen for human.